Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

Quadros, Rolen M.; Miura, Hiromi; Harms, Donald W.; Akatsuka, Hisako; Sato, Takehito; Aida, Tomomi; Redder, Ronald; Richardson, Guy P.; Inagaki, Yutaka; Sakai, Daisuke; Buckley, Shannon M.; Seshacharyulu, Parthasarathy; Batra, Surinder K.; Behlke, Mark A.; Zeiner, Sarah A.; Jacobi, Ashley M.; Izu, Yayoi; Thoreson, Wallace B.; Urness, Lisa D.; Mansour, Suzanne L.; Ohtsuka, Masato; Gurumurthy, Channabasavaiah B.

期刊名称Genome Biology
作者Quadros, Rolen M.; Miura, Hiromi; Harms, Donald W.; Akatsuka, Hisako; Sato, Takehito; Aida, Tomomi; Redder, Ronald; Richardson, Guy P.; Inagaki, Yutaka; Sakai, Daisuke; Buckley, Shannon M.; Seshacharyulu, Parthasarathy; Batra, Surinder K.; Behlke, Mark A.; Zeiner, Sarah A.; Jacobi, Ashley M.; Izu, Yayoi; Thoreson, Wallace B.; Urness, Lisa D.; Mansour, Suzanne L.; Ohtsuka, Masato; Gurumurthy, Channabasavaiah B.
卷第18卷
期第1期
电子ISSN1474-760X
文件大小1180.60KB
发表时间2017-12
DOI10.1186/s13059-017-1220-4

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Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins